1.25ml Ketamine + 0.5ml Atropine+ 0.25ml Diazepam + 5ml Physiologic Saline
Induction dose: 1.5-2 ml/rat IM
Middle laparothomy to expose the left kidney; Dissect the abdominal vessels in the following order letting ligatures prepared to knot it:
i. supra-renal aorta (3-0 ligature)ii. renal vein as closer as possible to the cavae (6-0 ligature) iii. infra-renal aorta (3-0 ligature to fix the canulla) iv. infra-renal aorta+cavae en bloc (3-0 ligature)
HEPARIN1000UI IV penile vein
URETER DISSECTIONOnce Heparin has been injected, dissect the ureter preserving its surrounding fat and divide it close to the bladder.
Dissect the kidney from its surrounding tissues
Knot the infra-renal aorta+cava en bloc (iv)
CANNULATION OF THE ABDOMINAL AORTA AND KIDNEY WASH.
Clamp the infra-renal aorta.
Insert and fix the cannula in the infra-renal aorta (iii).
Remove the vascular clamp.
Open the preservation solution flow (EuroCollins, 4ºC) and immediately interrupt the blood flow by knotting supra-renal aorta ligature (i).
Wash with a minimum volume of 1ml at a maximum speed of 20ml/h to avoid intra-renal pressure beyond 100 mm Hg.
EXCISIONTie the ligature of the renal vein (ii) and cut the renal vein as close as possible to the vena cava.
Once the kidney is washed, cut the renal artery with an aortic patch with the help of curved scissors. Note the shape and size of the aortic patch.
Store the kidney in preservation solution (EuroCollins) at 4ºC while preparing the recipient.